p38 α mapk14 protein  (BPS Bioscience)

Journal: Toxics

Article Title: Food Contaminants Effects on an In Vitro Model of Human Intestinal Epithelium

doi: 10.3390/toxics9060135

Figure Lengend Snippet: List of primers used in the study.

Article Snippet: , MAPK14 , Mitogen-activated protein kinase 14, also called p38-α , QT00079345 (QIAGEN).

Techniques:

Journal: Toxics

Article Title: Food Contaminants Effects on an In Vitro Model of Human Intestinal Epithelium

doi: 10.3390/toxics9060135

Figure Lengend Snippet: Effect of a 6 h incubation with the cocktail of cytokines on the relative mRNA expression of genes coding for the pro-inflammatory and pro-apoptotic signaling pathways measured by qPCR from the Caco-2/TC7 mono-culture ( a ) and from the Caco-2/TC7 and HT29-MTX co-culture ( b ). Data are expressed as the mean ± SEM ( n = 12). mRNA levels are expressed as fold induction over the CTL group set at 1. *, **** statistically significant difference vs. CTL ( p < 0.05; p < 0.0001, respectively). CK, cytokines; CTL, control; CASP3 , Caspase 3; CASP9 , Caspase 9; MAP3K1 , mitogen-activated protein kinase kinase kinase 1; MAPK8 , mitogen-activated protein kinase 8; MAPK14 , mitogen-activated protein kinase 14, also known as p38-α; MyD88 , myeloid differentiation primary response gene 88; STAT3 , signal transducer and activator of transcription 3; TNF-α , tumor necrosis factor α.

Article Snippet: , MAPK14 , Mitogen-activated protein kinase 14, also called p38-α , QT00079345 (QIAGEN).

Techniques: Incubation, Expressing, Co-Culture Assay

Journal: Toxics

Article Title: Food Contaminants Effects on an In Vitro Model of Human Intestinal Epithelium

doi: 10.3390/toxics9060135

Figure Lengend Snippet: Effect of a 6 h incubation with CML (300 µM) and/or CPF (300 µM) on the relative mRNA expression of genes coding for NF-κB and CXCL8 ( a ) measured by qPCR, and of IL-8 secretion measured by ELISA, in both the apical ( b ) and basal ( c ) media. Relative mRNA expression of genes involved in the pro-inflammatory and pro-apoptotic signaling pathways measured by qPCR from the Caco-2/TC7 & HT29-MTX co-culture ( d ). Data are expressed as the mean ± SEM ( n = 12). The level of gene expression is expressed as the fold induction over the CTL group set at 1. *, ** statistically significant difference ( p < 0.05; p < 0.01 CML vs. CTL); $$ statistically significant difference ( p < 0.01 CML vs. CPF/CML). CML, N (6)-Carboxymethyllysine; CPF, Chlorpyrifos; CTL, control; CASP3 , Caspase 3; CASP9 , Caspase 9; CXCL8 , Interleukin 8; MAP3K1 , mitogen-activated protein kinase 1; MAPK8 , mitogen-activated protein kinase 8; MAPK14 , mitogen-activated protein kinase 14, also called p38-α; MyD88 , myeloid differentiation primary response gene 88; NF-κB ; nuclear factor-kappa B; STAT3 , signal transducer and activator of transcription 3; TNF-α , tumor necrosis factor α.

Article Snippet: , MAPK14 , Mitogen-activated protein kinase 14, also called p38-α , QT00079345 (QIAGEN).

Techniques: Incubation, Expressing, Enzyme-linked Immunosorbent Assay, Co-Culture Assay

Journal: Toxics

Article Title: Food Contaminants Effects on an In Vitro Model of Human Intestinal Epithelium

doi: 10.3390/toxics9060135

Figure Lengend Snippet: Effect of a 6 h incubation with CML (300 µM) or acrylamide (5 mM) on relative mRNA expressions of genes coding for NF-κB and CXCL8 ( a ) measured by qPCR, and on IL-8 secretion measured by ELISA, in the apical ( b ) and the basal ( c ) media, and on the relative mRNA expression of genes coding for the pro-inflammatory and pro-apoptotic signaling pathways measured by qPCR ( d ) from the Caco-2/TC7 and HT29-MTX co-culture. Data were expressed as mean ± SEM ( n = 12). The level of gene expression was expressed as the fold induction over the CTL group set at 1. *, **, statistically significant difference ( p < 0.05; p < 0.01 vs. CTL); #, ## statistically significant difference ( p < 0.05; p < 0.01 vs. acrylamide). CML, N ε -Carboxymethyllysine; CTL, control CASP3 , Caspase 3; CASP9 , Caspase 9; CXCL8 , Interleukin 8; MAP3K1 , mitogen-activated protein kinase 1; MAPK8 , mitogen-activated protein kinase 8; MAPK14 , mitogen-activated protein kinase 14, also called p38-α; MyD88 , myeloid differentiation primary response gene 88; NF-κB , nuclear factor-kappa B; STAT3 , signal transducer and activator of transcription 3; TNF-α , tumor necrosis factors α.

Article Snippet: , MAPK14 , Mitogen-activated protein kinase 14, also called p38-α , QT00079345 (QIAGEN).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Expressing, Co-Culture Assay

Journal: Toxics

Article Title: Food Contaminants Effects on an In Vitro Model of Human Intestinal Epithelium

doi: 10.3390/toxics9060135

Figure Lengend Snippet: Potential effects of food contaminants on the co-culture of Caco-2/TC7 and HT29-MTX after a 6 h stimulation. CASP3 , Caspase 3; CASP9 , Caspase 9; CLDN4 , Claudin 4; CML, N(6)-Carboxymethyllysine; CPF, Chlorpyrifos; CTL, control; CXCL8 , Interleukin 8; MAP3K1 , mitogen-activated protein kinase 1; MAPK8 , mitogen-activated protein kinase 8; MAPK14 , mitogen-activated protein kinase 14, also called p38-α; MUC3 , Mucin 3; MUC5AC , Mucin 5AC; MyD88 , myeloid differentiation primary response gene 88; NF-κB , nuclear factor-kappa B; OCLN , Occludin; STAT3 , signal transducer and activator of transcription 3; TJP1 , tight junction protein 1; TNF-α , tumor necrosis factor α; VIL1 , Villin 1.

Article Snippet: , MAPK14 , Mitogen-activated protein kinase 14, also called p38-α , QT00079345 (QIAGEN).

Techniques: Co-Culture Assay